The roles of NF-κB and ROS in regulation of pro-inflammatory mediators of inflammation induction inLPS-stimulated zebrafish embryos

Study Summary

To our knowledge, this study is the first to examine this aspect of inflammation; we examined the roles of ROS and NF-κB in zebrafish embryos with inflammation induced by LPS using NAC and PDTC, specific inhibitors of ROS and NF-κB respectively. Zebrafish embryos treated with LPS had increases in NO, iNOS, COX-2, ROS, and NF-κB compared to the untreated control group. However, NAC and PDTC both inhibited production of NO and expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. In addition, NAC significantly inhibited production of ROS, and PDTC significantly attenuated mRNA expression of NF-κB. Taken together, these results suggest that LPS causes inflammation in zebrafish embryos by increasing inflammatory mediators (NO, iNOS, COX-2) via ROS and NF-κB regulation.

Expected Effects

The results of this study provide basic information on the mechanism of action of zebrafish inflammation induction and can be helpful in the screening of candidate functional foods and drugs for the treatment of inflammatory diseases.