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전주센터 세미나 개최 안내 (6. 28 (화)
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| 이름 : 최고관리자 | 작성일 : 2005.06.20 16:35 | 조회수 : 39271 |
| 전주센터에서 생리활성 물질 계측용 바이오칩 개발 연구와 관련하여 전문가를 초청하여 바이오칩 개발에 관한 세미나를 개최하오니 관심있는 분들의 많은 참석바랍니다. ■ 일 시 : 2005. 6. 28(화) 오후 4시 ■ 장 소 : 한국기초과학지원연구원 전주센터 세미나실 (전북대학교 농과대학 1호관) ■ 연사 및 세미나 주제 - 정형일 박사(연세대학교 생명공학과 조교수) ˝Nanoarrays of Biomolecules by Surfactant Activated Dip-Pen Nanolithography and Its Applications˝ ※ 문의 : 이해성 부장 (전주센터 분석연구부) 조선아 063) 270-4228 ◈ 세미나 초록 Nanoarrays of Biomolecules by Surfactant Activated Dip-Pen Nanolithography and Its Applications Arrays of biomolecules, such as, DNA and proteins, with well-defined feature size and spacing are important for studying surface-cellular interactions, drug discovery and disease diagnosis. Recently, there have been significant developments in the use of nanolithography techniques for patterning surfaces with proteins on the submicrometer length scale. In principle, one can use smaller sample volumes and achieve higher sensitivity due to the small size of the entire array and the individual features that comprise the array. Dip-pen nanolithography (DPN) is one technique that has shown particular promise in this area, allowing one to prepare standardized multicomponent arrays of biomolecules that can retain their biorecognition properties once transferred to a surface. DPN is a direct-write printing technique that uses chemically modified AFM tips to pattern materials on a variety of substrates at the nanoscale level. Direct nanoscale patterning of biotin on silanized glass using DPN was facilitated by the addition of a small amount of the biocompatible nonionic surfactant Tween-20. The fabrication of streptavidin or avidin nanostructures built upon DPN patterning of biotin represents a general route toward molecular recognition-mediated protein immobilization at the nanoscale due to the prevalence of biotin-tagged biomolecules. A correlation was found between activated biotin transfer from the AFM tip with surfactant and an increase in the wettability of the partially hydrophobic silanized substrate. Systematic variation of surfactant concentration represents a new control variable, which may expand the possible ink-substrate combinations used for patterning biotin and other biomolecules with DPN, including proteins. |
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