장비활용내용 < 800 MHz 핵자기공명분광기-질량분석기 시스템 < 선도연구장비

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800 MHz 핵자기공명분광기-질량분석기 시스템은 LC, NMR, MS가 온라인 연결된 hyphenated system으로 혼합물을 LC로 분리 후 고감도 800 MHz NMR로 화합물의 구조를 확인하는 대사체/천연물 분야 특화 운영 장비입니다.

• 대사체 및 천연물 연구 분야에서 대사물질 확인 및 대사기전 규명 등 다양한 분야에 적용 가능한 통합 분석 시스템
• 혼합물을 LC로 분리 후 고감도 800 MHz NMR과 UPLC-QTOF MS로 화합물의 구조 확인하는데 활용

막성사구체신염에서 fumarate의 역할

• 정상인과 미세변화증후군, 그리고 막성사구체신염을 가진 환자의 소변 내 대사물질 분석을 통해 질병 특이적인 마커로 fumarate 확인
• 막성사구체신염을 가진 환자들의 치료 반응에 따른 fumarate 농도 비교를 통해 질환과 관련이 높음을 확인
• 막성사구체신염 질환의 유무와 치료반응에 따른 fumarate의 유의적인 변화를 통해 질환에 대한 잠재적인 치료 타겟을 제시

(a) NMR을 이용한 혈청과 소변의 대사물질 다변량 분석 (b) 소변 대사물질과 관련된 대사물질 모식도

NMR을 이용한 소변 내 fumarate 농도 분석과 막성사구체 신염에서의 fumarate의 역할에 대한 모식도

• 통합 대사체 프로파일링 분석을 통한 바이오마커 탐지 및 대사 시그니처 탐색
• LC-SPE-NMR/MS hyphenated system을 활용한 천연물 혼합물 구조 확인

• Fumarate modulates phospholipase A2 receptor autoimmunity-induced podocyte injury in membranous nephropathy
  Hyung Ah Jo, Jin Seong Hyeon, Seung Hee Yang, Youngae Jung, Hunjoo Ha, Chang Wook Jeong, Cheol Kwak, Yaerim Kim, Hajeong Lee, Jung Pyo Lee, Kwon Wook Joo, Chun Soo Lim, Yon Su Kim, Geum-Sook Hwang, Dong Ki Kim,
  Kidney International, 2020, Online ahead of print, doi:10.1016/j.kint.2020.06.031
• A Variant of SLC1A5 Is a Mitochondrial Glutamine Transporter for Metabolic Reprogramming in Cancer Cells
  Hee Chan Yoo, Seung Joon Park, Miso Nam, Juwon Kang, Kibum Kim, Joo Hye Yeo, Joon-Ki Kim, Yunkyung Heo, Hee Seung Lee, Myeong Youl Lee, Chang Woo Lee, Jong Soon Kang, Yun-Hee Kim, Jinu Lee, Junjeong Choi, Geum-Sook Hwang, Seungmin Bang, Jung Min Han,
  Cell Metabolism, 2020, 31(2), PP 267-283, doi:10.1016/j.cmet.2019.11.020
• Glucose-dependent control of leucine metabolism by leucyl-tRNA synthetase 1
  Ina Yoon, Miso Nam, Hoi Kyoung Kim, Hee-Sun Moon, Sungmin Kim, Jayun Jang, Ji Ae Song, Seung Jae Jeong, Sang Bum Kim, Seongmin Cho, YounHa Kim, Jihye Lee, Won Suk Yang, Hee Chan Yoo, Kibum Kim, Min-Sun Kim, Aerin Yang, Kyukwang Cho, Hee-Sung Park, Geum-Sook Hwang, Kwang Yeon Hwang, Jung Min Han, Jong Hyun Kim, Sunghoon Kim,
  Science, 2020, 367(6474), PP 205-210, doi:10.1126/science.aau2753
• Monolayered g-C3N4 nanosheet as an emerging cationic building block for bifunctional 2D superlattice hybrid catalyst with controlled defect structures
  Nam Hee Kwon., Seung-Jae Shin, Xiaoyan Jin, Youngae Jiung, Geum-Sook Hwang, Hyungjun Kim, Seong-Ju Hwang,
  Applied Catalysis B: Environmental, 2020, 277, PP 119191 doi:10.1016/j.apcatb.2020.119191
• Eicosapentaenoic Acid (EPA) modulates glucose metabolism by targeting AMP-Activated Protein Kinase (AMPK) pathway
  Nami Kim, Mi Sun Kang, Miso Nam, Shin Ae Kim, Geum-Sook Hwang, Hyeon Soo Kim,
  International Journal of Molecular Sciences, 2019, 20(19), PP 4751, doi:10.3390/ijms20194751
• Urinary myo-inositol is associated with the clinical outcome in focal segmental glomerulosclerosis
  Jung Nam An, Jin Seong Hyeon, Youngae Jung, Young Wook Choi, Jin Hyuk Kim, Seung Hee Yang, Sohee Oh, Soie Kwon, Sang-Ho Lee, Jang-Hee Cho, Sun-Hee Park, Hunjoo Ha, Dong Ki Kim, Jung Pyo Lee, Geum-Sook Hwang,
  Scientific Reports, 2019, 9(1), PP 14707, doi:10.1038/s41598-019-51276-9
• Differential effects of typical Korean versus american-style diets on gut microbial composition and metabolic profile in healthy overweight koreans: A randomized crossover trial
  Ji-Hee Shin, Sunhee Jung, Seong-Ah Kim, Min-Sook Kang, Min-Sun Kim, Hyojee Joung, Geum-Sook Hwang, Dong-Mi Shin,
  Nutrients, 2019, 11(10), PP 2450, doi:10.3390/nu11102450
• Snail reprograms glucose metabolism by repressing phosphofructokinase PFKP allowing cancer cell survival under metabolic stress
  Nam Hee Kim, Yong Hoon Cha, Jueun Lee, Seon-Hyeong Lee, Ji Hye Yang, Jun Seop Yun, Eunae Sandra Cho, Xianglan Zhang, Miso Nam, Nami Kim, Young-Su Yuk, So Young Cha, Yoonmi Lee, Joo Kyung Ryu, Sunghyouk Park, Jae-Ho Cheong, Sang Won Kang, Soo-Youl Kim, Geum-Sook Hwang, Jong In Yook, Hyun Sil Kim,
  Nature Communications, 2017, 8, PP 14374, doi: 10.1038/ncomms14374
• The novel hypoxia-inducible factor-1α inhibitor IDF-11774 regulates cancer metabolism, thereby suppressing tumor growth
  Hyun Seoung Ban, Bo-Kyung Kim, Hongsub Lee, Hwan Mook Kim, Dipesh Harmalkar, Miso Nam, Song-Kyu Park, Kiho Lee, Joon-Tae Park, Inhyub Kim, Kyeong Lee, Geum-Sook Hwang, Misun Won,
  Cell Death and Disease, 2017, 8(6), PP e2843, doi: 10.1038/cddis.2017.235
• Piperine regulates UCP1 through the AMPK pathway by generating intracellular lactate production in muscle cells
  Nami Kim, Miso Nam, Mi Sun Kang, Jung Ok Lee, Young Woo Lee, Geum-Sook Hwang, Hyeon Soo Kim,
  Scientific Reports, 2017, 7, PP 41066, doi: 10.1038/srep41066
• Myocardial metabolic alterations in mice with diet-induced atherosclerosis: linking sulfur amino acid and lipid metabolism
  Jueun Lee, Sunhee Jung, Nami Kim, Min-Jeong Shin, Do Hyun Ryu, Geum-Sook Hwang,
  Scientific Reports, 2017, 7(1), 13597, doi: 10.1038/s41598-017-13991-z
• Software-assisted serum metabolite quantification using NMR
  Young-Sang Jung, Jin-Seong Hyeon, Geun-Sook Hwang,
  Analytica Chimica Acta, 2016, 934, PP 194-202, doi: 10.1016/j.aca.2016.04.054

• 주담당자 : 황금숙(gshwang@kbsi.re.kr/02-6908-6200)
• 부담당자 : 정영애(jya0819@kbsi.re.kr/02-6908-6233) / 이주은(lje3080@kbsi.re.kr/02-6908-6256)
• 주소 : 서울 서대문구 북아현로 150 한국기초과학지원연구원 서울서부센터 B107호