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High-Field Nuclear Magnetic Resonance

High-Field Nuclear Magnetic Resonance Leading Research Equipment Home

High Field-Nuclear Magnetic Resonance

Equipment Introduction

The high-field nuclear magnetic resonance instrument is core equipment for the study of biomolecular structures and the development of new drugs. It is installed at the Ochang Center and has been utilized as a national collaborative research instrument.

Equipment Characteristics

  • The sensitivity of the cryogenic probe to 1H is more than 4 times (8,000) that of conventional room-temperature probes; consequently, the experiment time can be shortened by a factor of 1/16
  • Protein structural studies can be performed with samples having a concentration of 100 μM or less, and the minimum mass for 13C experiments of natural products is approximately 100μg

Representative Research Case

Structural insights into the mechanism of activation of the TRPV1 channel by a membrane-bound tarantula toxin

  • TRPV1 is activated by a toxin called the double-knot toxin
  • It is necessary to reveal the tertiary structure of the double-knot toxin in order to identify the channel activation mechanism
  • For high-resoluton NMR spectra, 900-MHz NMR was used to perform 2D experiments such as 2D-NOESY to identify the exact tertiary structure
  • NMR solution structures of K1 and K2 (A,B).
  • NOESY-NMR spectra of K1 and K2 in solution.