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Bio High-Voltage Electron Microscope

Bio High-Voltage Electron Microscope Leading Research Equipment Home

Bio-High Voltage Electron Microscope

Equipment Introduction

The bio-HVEM has been in operation as national co-utilization equipment in basic and applied sciences for three-dimensional ultrastructure analysis of cell organelles, proteins, and bio-nano-specimens as well as the development of drugs and nanomaterials

Equipment Characteristics

  • 3D modeling of cell organelles by high tilting (± 70°) with high resolution (0.15 nm)
  • Enhanced high-contrast imaging using high-accelerating-voltage/in-column energy filter
  • Analysis of a large area with high resolution by a limitless panorama function
  • Cryo-EM analysis by the rapid and continuous freezing of biological specimens

Representative Research Case

Development of 3D ultrastructure analytical method for synaptic plasticity and synaptic vesicle

  • 3D modeling of the synaptic area between two neurons by EM based on a high-accelerating-voltage/panorama function to overcome the disadvantage of 2D imaging in a restricted area
  • Development of the analytical method can statistically quantify the distribution of synaptic vesicles in presynaptic neurons using a 3D model of the synapse
  • Identification/3D visualization of the differences of size and number of synaptic vesicles in presynaptic neuronal cell of the brain cortex between control and RF-835-MHz-exposed animals

3D ultrastructure analytical method for synaptic plasticity and synaptic vesicle
(a and b) 3D electron tomographic model of synapse and synaptic vesicles in brain cortex of control(a) and RF-835 exposed(b) animal(c) Statistical graphs showing the difference of synaptic vesicle distribution between control and RF-835 exposed animal in 3D volume