High-Field Nuclear Magnetic Resonance
High-Field Nuclear Magnetic Resonance Leading-edge Equipment Home
A 900 MHz nuclear magnetic resonance spectrometer, a core equipment for the study of biomolecular structure and development of new drugs, is installed at the Ochang Center. It is utilized as national collaborative research equipment.
Characteristics of the Equipment
- The sensitivity of the cryogenic probe to 1H is more than four times (8,000) that of conventional room temperature probes, so the experiment time can be shortened to 1/16
- Protein structural studies can be performed with 100-μM or lower concentration samples
- Minimum mass for 13C experiments on natural products is approximately 100 μg
Representative Research Case
Ligand mediated folding of the AbOmpA-PD
The folding of AbOmpA-PD is mediated by the ligand binding. AbOmpA-PD exists as a mixture of partially folded forms in the absence of the ligand. Using 900-MHz NMR, a 15N-relaxation dispersion CPMG experiment reveals the presence of millisecond timescale motion of the apo-state, and the molecular mechanism of the ligand-mediated folding of AbOmpA-PD.
Analysis of milli-second time-scale motion of AbOmpA-PD in the apo-state II
- Analysis of protein structure and interactions
- Self-assembly compound analysis