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High-Field Nuclear Magnetic Resonance

High-Field Nuclear Magnetic Resonance Leading-edge Equipment Home

Equipment Introduction

900 MHz Nuclear Magnetic Resonance Spectrometer is a core equipment for the study of biomolecular structure and development of new drugs which is installed in Ochang Center. It is utilized as a national collaborative research equipment.

Characteristics of the Equipment

  • The sensitivity of the cryogenic probe to 1H is more than 4 times (8,000) that of conventional room temperature probe, so the experiment time can be shortened to 1/16
  • Protein structural studies can be performed with 100 μM or lower concentration samples
  • Minimum mass for 13C experiment of natural products is about 100 μg

Representative Research Case

Target Search Pathways Visualized by Paramagnetic NMR Spectroscopy

NMR paramagnetic relaxation enhancement have been employed to visualize the encounter complexes between histidine-containing phosphocarrier protein and the N-terminal domain of enzyme I and demonstrate that protein association can be significantly enhanced by engineering on-pathways.

Paramagnetic NMR spectroscopy visualizes the protein target search process

Application

  • J-based configuration analysis of natural compound
  • Self assembly and DOSY NMR of the molecular knot and macrocycle

Representative Research Papers

  • Single-Molecule-Based Detection of Conserved Influenza A Virus RNA Promoter Using a Protein Nanopore
    Sohee Oh, Mi-Kyung Lee, Seung-Wook Chi
    ACS Sensors, 2019, 4(11), pp 2849-2853, doi: 10.1021/acssensors.9b01558.
  • NMR Investigation of the Interaction between the RecQ C-Terminal Domain of Human Bloom Syndrome Protein and G-Quadruplex DNA from the Human c-Myc Promoter
    SungjinLee, Ae ReeLee, Kyoung SeokRyu, Joon HwaLee, Chin JuPark,
    J Mol Biol. 2019, 431(4), pp 794-806. doi: 10.1016/j.jmb.2019.01.010
  • WS9326H, an Antiangiogenic Pyrazolone-Bearing Peptide from an Intertidal Mudflat Actinomycete
    MunhyungBae, JedoOh, Eun SeoBae, JoonseokOh, JoonseongHur, Young GerSuh, Sang KookLee, JongheonShin, Dong ChanOh,
    Org Lett. 2018, 20(7), pp 1999-2002, doi: 10.1021/acs.orglett.8b00546.
  • Dimeric- and trimeric sesquiterpenes from the flower of Inula japonica
    Jin Q., Lee J.W., Jang H., Lee H.L., Kim J.G., Wu W., Lee D., Kim E.-H., Kim Y., Hong J.T., Lee M.K., Hwang B.Y.,
    Phytochemistry, 2018, 155 (1), pp 107-113, doi:10.1016/j.phytochem.2018.07.008
  • Facilitated Protein Association via Engineered Target Search Pathways Visualized by Paramagnetic NMR Spectroscopy
    An S.Y., Kim E.-H., Suh J.-Y.,
    Structure, 2018, 26 (6), pp 887-89300, doi:10.1016/j.str.2018.04.009
  • Diverse Metabolite Variations in Tea (Camellia sinensis L.) Leaves Grown under Various Shade Conditions Revisited: A Metabolomics Study
    Ji H.-G., Lee Y.-R., Lee M.-S., Hwang K.H., Park C.Y., Kim E.-H., Park J.S., Hong Y.-S.,
    Journal of Agricultural and Food Chemistry, 2018, 66 (8), pp 1889-1897, doi:10.1021/acs.jafc.7b04768
  • Coordination-Driven Self-Assembly of Heterotrimetallic Barrel and Bimetallic Cages Using a Cobalt Sandwich-Based Tetratopic Donor
    Singh N., Singh J., Kim D., Kim D.H., Kim E.-H., Lah M.S., Chi K.-W.,
    Inorganic Chemistry, 2018, 57 (7), pp 3521-3528, doi:10.1021/acs.inorgchem.7b02653
  • Determination of the Absolute Configuration of Polyhydroxy Compound Ostreol B Isolated from the Dinoflagellate Ostreopsis cf. ovata
    Hwang B.S., Yoon E.Y., Jeong E.J., Park J., Kim E.-H., Rho J.-R.,
    Journal of Organic Chemistry, 2018, 83 (1), pp 194-202, doi:10.1021/acs.joc.7b02569
  • Evidence of link between quorum sensing and sugar metabolism in Escherichia coli revealed via cocrystal structures of LsrK and HPr
    Ha J.-H., Hauk P., Cho K., Eo Y., Ma X., Stephens K., Cha S., Jeong M., Suh J.-Y., Sintim H.O., Bentley W.E., Ryu K.-S.,
    Science Advances, 2018, 4 (6), eaar7063, doi:10.1126/sciadv.aar7063
  • Zinc-mediated Reversible Multimerization of Hsp31 Enhances the Activity of Holding Chaperone
    Kim J., Choi D., Cha S.-Y., Oh Y.-M., Hwang E., Park C., Ryu K.-S.,
    Journal of Molecular Biology, 2018, 430 (12), pp 1760-1772, doi:10.1016/j.jmb.2018.04.029
  • Investigation of cationicity and structure of pseudin-2 analogues for enhanced bacterial selectivity and anti-inflammatory activity
    Jeon D., Jeong M.-C., Jacob B., Bang J.K., Kim E.-H., Cheong C., Jung I.D., Park Y., Kim Y.,
    Scientific Reports, 2017, 7 (1), 1455, doi:10.1038/s41598-017-01474-0
  • Amphiphilic Peptide Nanorods Based on Oligo-Phenylalanine as a Biocompatible Drug Carrier
    Song S.J., Lee S., Ryu K.-S., Choi J.S.,
    Bioconjugate Chemistry, 2017, 28 (9), pp 2266-2276, doi:10.1021/acs.bioconjchem.7b00247
  • Metabolic phenotyping of various tea (Camellia sinensis L.) cultivars and understanding of their intrinsic metabolism
    Hyang-Gi Ji, Yeong-Ran Lee, Min-Seuk Lee, Kyeong Hwan Hwang, Eun-Hee Kim, Jun Seong Park, Young-Shick Hong
    Food Chemistry vol. 233, 321-330 (2017), doi: 10.1016/j.foodchem.2017.04.079