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Equipment Introduction

Bio-HVEM has been in operation as a national co-utilization equipment in basic and applied sciences for 3-dimensional large area-high resolution structural analysis of cell organelles, proteins, and bio-nano specimens as well as development of drug and nano materials.

Characteristics of the Equipment

• 3D modeling of cell organelles by high tilting (±70°) and high resolution (0.15 nm)
• Enhanced contrast imaging using high accelerating voltage / in-column energy filter
• Analysis of Enhanced large-area with high resolution by Limitless panorama function
• Cryo-EM analysis by rapid and continuous freezing of biological specimen

Representative Research Case

3D Reconstruction and modeling of helical assembly of peptide programmable nanoparticles by Bio-HVEM analytical system

3D reconstruction of Peptide programmable nanoparticle superstructure through Bio-high voltage electron microscope (Bio-HVEM) system and 3D Electron tomography with high-resolution and high-tilting angle.

The tilt series of AuPt/HC/SWNT superstructure images were recorded with an interval of 2° by electron tomography of Bio-HVEM. 3D tomogram and model were sequentially reconstructed from aligned tilt series images

Application

• 3D ultrastructural analysis of thick(>1 μm) bio-nano materials using electron tomography
• 3D large area analysis of bio-nano materials using limitless panorama

Representative Research Papers

• Intracellular formyl peptide receptor regulates naïve CD4 T cell migration
  Lee HY, Jeong YS, Lee M, Kweon HS, Huh YH, Park JS, Hwang JE, Kim K, Bae YS
  Article in Biochemical and Biophysical Research Communications, 497(1), 2018, doi: 10.1016/j.bbrc.2018.02.060
• Exposure to 835 MHz radiofrequency electromagnetic field induces autophagy in hippocampus but not in brain stem of mice.
  Kim JH, Yu DH, Kim HJ, Huh YH, Cho SW, Lee JK, Kim HG, Kim HR,
  Toxicol Ind Health, 34(1), 23-35 (2018), doi: 10.1177/0748233717740066
• Changes in numbers and size of synaptic vesicles of cortical neurons induced by exposure to 835 MHz radiofrequency-electromagnetic field
  Ju Hwan Kim , Hyo-Jeong Kim , Da-Hyeon Yu, Hee-Seok Kweon, Yang Hoon Huh , Hak Rim Kim
  Published: October 18, (2017) doi: 10.1371/journal.pone.0186416
• Early Pheromone Experience Modifies a Synaptic Activity to Influence Adult Pheromone Responses of C. elegans
  Hong M, Ryu L, Ow MC, Kim J, Je AR, Chinta S, Huh YH, Lee KJ, Butcher RA, Choi H, Sengupta P, Hall SE, Kim K,
  Curr Biol. 27(20), 3168-3177 (2017), doi: 10.1016/j.cub.2017.08.068.
• Development of LC3/GABARAP sensors containing a LIR and a hydrophobic domain to monitor autophagy
  You‐Kyung Lee, Yong‐Woo Jun, Ha‐Eun Choi, Yang Hoon Huh, Bong‐Kiun Kaang, Deok‐Jin Jang, Jin‐A Lee,
  The EMBO Journal, 36, 1100-1116 (2017), doi: 10.15252/embj.201696315
• Long-term exposure to 835 MHz RF-EMF induces hyperactivity, autophagy and demyelination in the cortical neurons of mice
  Ju Hwan Kim,Da-Hyeon Yu, Yang Hoon Huh, Eun Ho Lee, Hyung-Gun Kim & Hak Rim Kim,
  Scientific Reports 7, Article number: 41129 (2017), doi: 10.1038/srep41129
• Pyruvate dehydrogenase kinase 4 deficiency attenuates cisplatin-induced acute kidney injury
  Chang Joo Oh, Chae-Myeong Ha, Young-Keun Choi, Sungmi Park, Mi Sun Choe, Nam Ho Jeoung, Yang Hoon Huh, Hyo-Jeong Kim, Hee-Seok Kweon, Ji-min Lee, Sun Joo Lee, Jae-Han Jeon, Robert A. Harris, Keun-Gyu Park, In-Kyu Lee,
  Kidney International vol. 91, 880-895 (2017), doi: 10.1016/j.kint.2016.10.011
• Induction of Autophagy in the Striatum and Hypothalamus of Mice after 835 MHz Radiofrequency Exposure
  Ju Hwan Kim, Yang Hoon Huh, Hak Rim Kim,
  PLoS One. 11(4), e0153308 (2016), doi: 10.1371/journal.pone.0153308
• Soft matter DNA nanoparticles hybridized with CpG motifs and peptide nucleic acids enable immunological treatment of cancer
  Jin J.-O., Kim H., Huh Y.H., Herrmann A., Kwak M.,
  Journal of Controlled Release, 2019, 315(), PP 76-84, doi:10.1016/j.jconrel.2019.09.013
• PDK4 augments ER–mitochondria contact to dampen skeletal muscle insulin signaling during obesity
  Thoudam T., Ha C.-M., Leem J., Chanda D., Park J.-S., Kim H.-J., Jeon J.-H., Choi Y.-K., Liangpunsakul S., Huh Y.H., Kwon T.-H., Park K.-G., Harris R.A., Park K.-S., Rhee H.-W., Lee I.-K.,
  Diabetes, 2019, 68(3), PP 571-586, doi:10.2337/db18-0363
• Early exposure to radiofrequency electromagnetic fields at 1850 MHz affects auditory circuits in early postnatal mice
  Kim J.H., Huh Y.H., Lee J.-H., Jung J.Y., Ahn S.C., Kim H.R.,
  Scientific Reports, 2019, 9(1), PP -, doi:10.1038/s41598-018-36868-1
• Diverse Structural Conversion and Aggregation Pathways of Alzheimer's Amyloid-β (1-40)
  Lin Y., Sahoo B.R., Ozawa D., Kinoshita M., Kang J., Lim M.H., Okumura M., Huh Y.H., Moon E., Jang J.H., Lee H.-J., Ryu K.-Y., Ham S., Won H.-S., Ryu K.-S., Sugiki T., Bang J.K., Hoe H.-S., Fujiwara T., Ramamoorthy A., Lee Y.-H.,
  ACS Nano, 2019, 13(8), PP 8766-8783, doi:10.1021/acsnano.9b01578
• Haploinsufficiency of Cyfip2 Causes Lithium-Responsive Prefrontal Dysfunction
  Seung-Hyun Lee, Yinhua Zhang, Jina Park, Bowon Kim, Yangsik Kim, Sang Hoon Lee, Gyu Hyun Kim, Yang Hoon Huh, Bokyoung Lee, Yoonhee Kim, Yeunkum Lee, Jin Yong Kim,Hyojin Kang, Su-Yeon Choi,Seil Jang, Yan Li, Shinhyun Kim, Chunmei Jin, Kaifang Pang, Eunjeong Kim, Yoontae Lee, Hyun Kim, Eunjoon Kim, Jee Hyun Choi, eongjin Kim, Kea Joo Lee, Se-Young Choi, and Kihoon Han,
  Ann. Neurol., 2020, 88:526-543, doi:10.1002/ana.25827
• Mitochondrial carnitine palmitoyltransferase 2 is involved in Nε-(carboxymethyl)-lysine-mediated diabetic nephropathy.
  Jangho Leea, Ju-Yong Hyonb, Jin Young Minc, Yang Hoon Huh, Hyo Jung Kim, Hayoung Leec, Sung Ho Yun, Chi-Won Choi, Su Jeong Ha, Joon Parka, Young-Ho Chung, Hye Gwang Jeong, Sang Keun Ha, Sung Keun Jung, YoonSook Kim, Eun Hee Han,
  Parmacol. Res., 2020, doi: https://doi.org/10.1016/j.phrs.2019.104600