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Bio High-Voltage Electron Microscope

Bio High-Voltage Electron Microscope Leading-edge Equipment Home

Equipment Introduction

Bio-HVEM has been in operation as a national co-utilization equipment in basic and applied sciences for three-dimensional large-area-high-resolution structural analysis of cell organelles, proteins, and bio-nano specimens, as well as development of drugs and nano materials.

Characteristics of the Equipment

  • 3-D modeling of cell organelles by high tilting (±70°) and high resolution (0.15 nm)
  • Enhanced high contrast imaging using high accelerating voltage/in-column energy filter
  • Analysis of enhanced large-area with high resolution by limitless panorama function
  • Cryo-EM analysis by rapid and continuous freezing of biological specimen

Representative Research Case

3D Reconstruction and modeling of helical assembly of peptide programmable nanoparticles by electron tomography with high-resolution and -tilting angle

Biomaterials derived via programmable supramolecular protein assembly provide a viable means of constructing precisely defined structures. We present programmed superstructures of AuPt nanoparticles (NPs) on carbon nanotubes (CNTs) that exhibit distinct electrocatalytic activities. Surface cysteine residues of the peptides create AuPt-specific nucleation site, which allow for precise positioning of NPs onto helical geometries, as confirmed by 3-D reconstruction. This origami research with 3D tilting electron tomography function of Bio-HVEM for design and confirmation approach can be expanded to versatile fields to build sophisticated functional assemblies.

Electron tomography and 3D reconstruction of Peptide programmable nanoparticle superstructure through Bio-high voltage electron microscope (Bio-HVEM). The tilt series of AuPt/HC/SWNT superstructure images were recorded over a tilt range of -50° to +50°, with an interval of 2°. 3-D surface renderings were reconstructed from the 3D tomogram which generated by alignment of digitized tilt series images.

Application

  • 3-D ultrastructural analysis of cellular organelles using electron tomography
  • 3-D large-area analysis of biological tissues using limitless panorama