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Bio High-Voltage Electron Microscope

Bio High-Voltage Electron Microscope Leading-edge Equipment Home

Equipment Introduction

Bio-HVEM has been in operation as a national co-utilization equipment in basic and applied sciences for three-dimensional large-area-high-resolution structural analysis of cell organelles, proteins, and bio-nano specimens, as well as development of drugs and nano materials.

Characteristics of the Equipment

  • 3-D modeling of cell organelles by high tilting (±70°) and high resolution (0.15 nm)
  • Enhanced high contrast imaging using high accelerating voltage/in-column energy filter
  • Analysis of enhanced large-area with high resolution by limitless panorama function
  • Cryo-EM analysis by rapid and continuous freezing of biological specimen

Representative Research Case

3D Reconstruction and modeling of helical assembly of peptide programmable nanoparticles by electron tomography with high-resolution and -tilting angle

Biomaterials derived via programmable supramolecular protein assembly provide a viable means of constructing precisely defined structures. We present programmed superstructures of AuPt nanoparticles (NPs) on carbon nanotubes (CNTs) that exhibit distinct electrocatalytic activities. Surface cysteine residues of the peptides create AuPt-specific nucleation site, which allow for precise positioning of NPs onto helical geometries, as confirmed by 3-D reconstruction. This origami research with 3D tilting electron tomography function of Bio-HVEM for design and confirmation approach can be expanded to versatile fields to build sophisticated functional assemblies.

Electron tomography and 3D reconstruction of Peptide programmable nanoparticle superstructure through Bio-high voltage electron microscope (Bio-HVEM). The tilt series of AuPt/HC/SWNT superstructure images were recorded over a tilt range of -50° to +50°, with an interval of 2°. 3-D surface renderings were reconstructed from the 3D tomogram which generated by alignment of digitized tilt series images.


  • 3-D ultrastructural analysis of cellular organelles using electron tomography
  • 3-D large-area analysis of biological tissues using limitless panorama

Representative Research Papers

  • Peroxiredoxin5 Controls Vertebrate Ciliogenesis by Modulating Mitochondrial Reactive Oxygen Species
    Ji, Y.R., Chae, S.M., Lee, H.K., Park, I.J., Kim, C.W., Ismail, T., Kim, Y., Park, J.W., Kwon, O.S., Kang, B.S., Lee,  D.S., Bae, J.S., Kim, S.H., Moon, P.G., Baek, M.C., Park, M.J., Kil, I.S., Rhee, S.G., Kim, J., Huh, Y.H., Shin, J.Y., Min, K.J., Kwon, T.K., Jang, D.G., Woo, H.A., Kwon, T.J., Park, T.J., Lee, H.S., Antioxidants & Redox Signaling, 2019, 30 (14), doi:10.1089/ars.2018.7507
  • Early exposure to radiofrequency electromagnetic fields at 1850 MHz affects auditory circuits in early postnatal mice
    Kim, J.H., Huh, Y.H., Lee, J.-H., Jung, J.Y., Ahn, S.C., Kim, H.R., Scientific Reports, 2019, 9 (1), pp -, doi:10.1038/s41598-018-36868-1
  • Bioaccumulation of polystyrene nanoplastics and their effect on the toxicity of Au ions in zebrafish embryos
    Lee, W.S., Cho, H.-J., Kim, E., Huh, Y.H., Kim, H.-J., Kim, B., Kang, T., Lee, J.-S., Jeong, J., Nanoscale, 2019, 11 (7), pp 3200-3207, doi:10.1039/c8nr09321k
  • PDK4 Augments ER–Mitochondria Contact to Dampen Skeletal Muscle Insulin Signaling During Obesity
    Thoudam, T., Ha, C.-M., Leem, J., Chanda, D., Park, J.-S., Kim, H.-J., Jeon, J.-H., Choi, Y.-K., Liangpunsakul, S., Huh, Y.H., Kwon, T.-H., Park, K.-G., Harris, R.A., Park, K.-S., Rhee, H.-W., Lee, I.-K., Diabetes, 2019, 68 (3), pp 571-586, doi:10.2337/db18-0363
  • Peptide-Programmable Nanoparticle Superstructures with Tailored Electrocatalytic Activity
    Kang, E.S., Kim, Y.-T., Ko, Y.-S., Kim, N.H., Cho, G., Huh, Y.H., Kim, J.-H., Nam, J., Thach, T.T., Youn, D., Kim, Y.D., Yun, W.S., Degrado, W.F., Kim, S.Y., Hammond, P.T., Lee, J., Kwon, Y.-U., Ha, D.-H., Kim, Y.H., ACS Nano, 2018, 12 (7), pp 6554-6562, doi:10.1021/acsnano.8b01146
  • Feeding state regulates pheromone-mediated avoidance behavior via the insulin signaling pathway in Caenorhabditis elegans
    Ryu, L., Cheon, Y., Huh, Y.H., Pyo, S., Chinta, S., Choi, H., Butcher, R.A., Kim, K., EMBO Journal, 2018, 37 (15), pp -, doi:10.15252/embj.201798402
  • Construction of a 3D mammary duct based on spatial localization of the extracellular matrix
    Cho, Y., Moon, W.K., Kim, H.S., Na, K., Yang, J.H., Huh, Y.H., Kim, J.A., Chung, S., Lee, S.H., NPG Asia Materials, 2018, 10 (10), pp 970-981, doi:10.1038/s41427-018-0089-3
  • Changes in numbers and size of synaptic vesicles of cortical neurons induced by exposure to 835 MHz radiofrequency-electromagnetic field
    Ju Hwan Kim , Hyo-Jeong Kim , Da-Hyeon Yu, Hee-Seok Kweon, Yang Hoon Huh , Hak Rim Kim Published: October 18, (2017) doi: 10.1371/journal.pone.0186416
  • Early Pheromone Experience Modifies a Synaptic Activity to Influence Adult Pheromone Responses of C. elegans
    Hong M, Ryu L, Ow MC, Kim J, Je AR, Chinta S, Huh YH, Lee KJ, Butcher RA, Choi H, Sengupta P, Hall SE, Kim K, Curr Biol. 27(20), 3168-3177 (2017), doi: 10.1016/j.cub.2017.08.068
  • Development of LC3/GABARAP sensors containing a LIR and a hydrophobic domain to monitor autophagy
    You‐Kyung Lee, Yong‐Woo Jun, Ha‐Eun Choi, Yang Hoon Huh, Bong‐Kiun Kaang, Deok‐Jin Jang, Jin‐A Lee, The EMBO Journal, 36, 1100-1116 (2017), doi: 10.15252/embj.201696315
  • Long-term exposure to 835 MHz RF-EMF induces hyperactivity, autophagy and demyelination in the cortical neurons of mice
    Ju Hwan Kim,Da-Hyeon Yu, Yang Hoon Huh, Eun Ho Lee, Hyung-Gun Kim & Hak Rim Kim, Scientific Reports 7, Article number: 41129 (2017), doi: 10.1038/srep41129