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SPE-800-MHz NMR-MS System

SPE-800-MHz NMR-MS System Leading-edge Equipment Home

Equipment Introduction

SPE-800 MHz NMR-MS System is a hyphenated system connected by LC, NMR, and MS. It is a specialized operation equipment for metabolites/natural products which identify the structure of compounds by high sensitivity Cryogenic 800 MHz NMR after separating the mixture by LC.

Characteristics of the Equipment

  • It is used as an integrated analysis system in various fields such as metabolite identification and metabolism elucidation in metabolomics and natural product research.
  • This equipment is used to identify of the structure of compounds by high sensitivity 800 MHz NMR and UPLC-QTOF MS after separating the mixture by LC.

Representative Research Case

Metabolic changes of mouse urine and serum during progression of diabetic kidney disease

  • We observed the specific changes of urine and serum metabolites in mouse during progression of diabetic kidney disease (DKD).
  • This study presented potential biomarkers for DKD through significant alterations in metabolites during progression of DKD

(a) Multivariate analysis of serum and urine metabolite using NMR (b) Pathway of metabolites related to changes in urine

Application

  • Metabolic biomarker and signature discovery by integrated metabolic profiling analysis
  • Identification of natural product mixture using LC-SPENMR/ MS hyphenated system

Representative Research Papers

  • Fumarate modulates phospholipase A2 receptor autoimmunity-induced podocyte injury in membranous nephropathy
    Hyung Ah Jo, Jin Seong Hyeon, Seung Hee Yang, Youngae Jung, Hunjoo Ha, Chang Wook Jeong, Cheol Kwak, Yaerim Kim, Hajeong Lee, Jung Pyo Lee, Kwon Wook Joo, Chun Soo Lim, Yon Su Kim, Geum-Sook Hwang, Dong Ki Kim,
    Kidney International, 2020, Online ahead of print, doi:10.1016/j.kint.2020.06.031
  • A Variant of SLC1A5 Is a Mitochondrial Glutamine Transporter for Metabolic Reprogramming in Cancer Cells
    Hee Chan Yoo, Seung Joon Park, Miso Nam, Juwon Kang, Kibum Kim, Joo Hye Yeo, Joon-Ki Kim, Yunkyung Heo, Hee Seung Lee, Myeong Youl Lee, Chang Woo Lee, Jong Soon Kang, Yun-Hee Kim, Jinu Lee, Junjeong Choi, Geum-Sook Hwang, Seungmin Bang, Jung Min Han,
    Cell Metabolism, 2020, 31(2), PP 267-283, doi:10.1016/j.cmet.2019.11.020
  • Glucose-dependent control of leucine metabolism by leucyl-tRNA synthetase 1
    Ina Yoon, Miso Nam, Hoi Kyoung Kim, Hee-Sun Moon, Sungmin Kim, Jayun Jang, Ji Ae Song, Seung Jae Jeong, Sang Bum Kim, Seongmin Cho, YounHa Kim, Jihye Lee, Won Suk Yang, Hee Chan Yoo, Kibum Kim, Min-Sun Kim, Aerin Yang, Kyukwang Cho, Hee-Sung Park, Geum-Sook Hwang, Kwang Yeon Hwang, Jung Min Han, Jong Hyun Kim, Sunghoon Kim,
    Science, 2020, 367(6474), PP 205-210, doi:10.1126/science.aau2753
  • Monolayered g-C3N4 nanosheet as an emerging cationic building block for bifunctional 2D superlattice hybrid catalyst with controlled defect structures
    Nam Hee Kwon., Seung-Jae Shin, Xiaoyan Jin, Youngae Jiung, Geum-Sook Hwang, Hyungjun Kim, Seong-Ju Hwang,
    Applied Catalysis B: Environmental, 2020, 277, PP 119191 doi:10.1016/j.apcatb.2020.119191
  • Eicosapentaenoic Acid (EPA) modulates glucose metabolism by targeting AMP-Activated Protein Kinase (AMPK) pathway
    Nami Kim, Mi Sun Kang, Miso Nam, Shin Ae Kim, Geum-Sook Hwang, Hyeon Soo Kim,
    International Journal of Molecular Sciences, 2019, 20(19), PP 4751, doi:10.3390/ijms20194751
  • Urinary myo-inositol is associated with the clinical outcome in focal segmental glomerulosclerosis
    Jung Nam An, Jin Seong Hyeon, Youngae Jung, Young Wook Choi, Jin Hyuk Kim, Seung Hee Yang, Sohee Oh, Soie Kwon, Sang-Ho Lee, Jang-Hee Cho, Sun-Hee Park, Hunjoo Ha, Dong Ki Kim, Jung Pyo Lee, Geum-Sook Hwang,
    Scientific Reports, 2019, 9(1), PP 14707, doi:10.1038/s41598-019-51276-9
  • Differential effects of typical Korean versus american-style diets on gut microbial composition and metabolic profile in healthy overweight koreans: A randomized crossover trial
    Ji-Hee Shin, Sunhee Jung, Seong-Ah Kim, Min-Sook Kang, Min-Sun Kim, Hyojee Joung, Geum-Sook Hwang, Dong-Mi Shin,
    Nutrients, 2019, 11(10), PP 2450, doi:10.3390/nu11102450
  • Snail reprograms glucose metabolism by repressing phosphofructokinase PFKP allowing cancer cell survival under metabolic stress
    Nam Hee Kim, Yong Hoon Cha, Jueun Lee, Seon-Hyeong Lee, Ji Hye Yang, Jun Seop Yun, Eunae Sandra Cho, Xianglan Zhang, Miso Nam, Nami Kim, Young-Su Yuk, So Young Cha, Yoonmi Lee, Joo Kyung Ryu, Sunghyouk Park, Jae-Ho Cheong, Sang Won Kang, Soo-Youl Kim, Geum-Sook Hwang, Jong In Yook, Hyun Sil Kim,
    Nature Communications, 2017, 8, PP 14374, doi: 10.1038/ncomms14374
  • The novel hypoxia-inducible factor-1α inhibitor IDF-11774 regulates cancer metabolism, thereby suppressing tumor growth
    Hyun Seoung Ban, Bo-Kyung Kim, Hongsub Lee, Hwan Mook Kim, Dipesh Harmalkar, Miso Nam, Song-Kyu Park, Kiho Lee, Joon-Tae Park, Inhyub Kim, Kyeong Lee, Geum-Sook Hwang, Misun Won,
    Cell Death and Disease, 2017, 8(6), PP e2843, doi: 10.1038/cddis.2017.235
  • Piperine regulates UCP1 through the AMPK pathway by generating intracellular lactate production in muscle cells
    Nami Kim, Miso Nam, Mi Sun Kang, Jung Ok Lee, Young Woo Lee, Geum-Sook Hwang, Hyeon Soo Kim,
    Scientific Reports, 2017, 7, PP 41066, doi: 10.1038/srep41066
  • Myocardial metabolic alterations in mice with diet-induced atherosclerosis: linking sulfur amino acid and lipid metabolism
    Jueun Lee, Sunhee Jung, Nami Kim, Min-Jeong Shin, Do Hyun Ryu, Geum-Sook Hwang,
    Scientific Reports, 2017, 7(1), 13597, doi: 10.1038/s41598-017-13991-z
  • Software-assisted serum metabolite quantification using NMR
    Young-Sang Jung, Jin-Seong Hyeon, Geun-Sook Hwang,
    Analytica Chimica Acta, 2016, 934, PP 194-202, doi: 10.1016/j.aca.2016.04.054