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SPE-800-MHz NMR-MS System

SPE-800-MHz NMR-MS System Leading-edge Equipment Home

Equipment Introduction

The SPE-800-MHz NMR-MS system is a hyphenated system connected by LC, NMR, and MS. It is a specialized operational equipment for metabolites/natural products and identifies the structure of compounds by high-sensitivity cryogenic 800-MHz NMR after separating the mixture by means of LC.

Characteristics of the Equipment

  • This integrated analysis system is used in various fields, such as metabolite identification and metabolism elucidation in metabolomics and natural materials research.
  • It is used to identify the structure of the compounds by means of high-sensitivity cryogenic 800-MHz NMR and UPLC-QTOF MS after separating the mixture by LC.

Representative Research Case

Snail reprograms glucose metabolism by repressing phosphofructokinase PFKP, allowing cancer cell survival under metabolic stress

We showed that Snail (SNAI1) is a key transcriptional repressor of the epithelial–mesenchymal transition (EMT). It regulates glucose flux toward the pentose phosphate pathway (PPP), allowing cancer cell survival under metabolic stress.

A diagram and quantification of the changes in intracellular metabolites following inducible knockdown of PFKP in MDA-MB-231 cells.

Application

  • Metabolomic biomarker and signature discovery by integrated metabolic profiling analysis
  • Identification of mixture using LC-SPE-NMR/MS hyphenated system

Representative Research Papers

  • Myocardial metabolic alterations in mice with diet-induced atherosclerosis: linking sulfur amino acid and lipid metabolism
    Jueun Lee, Sunhee Jung, Nami Kim, Min-Jeong Shin, Do Hyun Ryu, Geum-Sook Hwang.
    Scientific Reports vol. 7, 13597 (2017), doi: 10.1038/s41598-017-13991-z
  • Piperine regulates UCP1 through the AMPK pathway by generating intracellular lactate production in muscle cells
    Nami Kim, Miso Nam, Mi Sun Kang, Jung Ok Lee, Young Woo Lee, Geum-Sook Hwang, Hyeon Soo Kim.
    Scientific Reports vol. 7, 41066 (2017), doi: 10.1038/srep41066
  • The novel hypoxia-inducible factor-1α inhibitor IDF-11774 regulates cancer metabolism, thereby suppressing tumor growth
    Hyun Seoung Ban, Bo-Kyung Kim, Hongsub Lee, Hwan Mook Kim, Dipesh Harmalkar, Miso Nam, Song-Kyu Park, Kiho Lee, Joon-Tae Park, Inhyub Kim, Kyeong Lee, Geum-Sook Hwang, Misun Won.
    Cell Death and Disease vol. 8, e2843 (2017), doi: 10.1038/cddis.2017.235
  • Snail reprograms glucose metabolism by repressing phosphofructokinase PFKP allowing cancer cell survival under metabolic stress
    Nam Hee Kim, Yong Hoon Cha, Jueun Lee, Seon-Hyeong Lee, Ji Hye Yang, Jun Seop Yun, Eunae Sandra Cho, Xianglan Zhang, Miso Nam, Nami Kim, Young-Su Yuk, So Young Cha, Yoonmi Lee, Joo Kyung Ryu, Sunghyouk Park, Jae-Ho Cheong, Sang Won Kang, Soo-Youl Kim, Geum-Sook Hwang, Jong In Yook & Hyun Sil Kim
    Nature Communications 8, Article number: 14374 (2017), doi: 10.1038/ncomms14374
  • Software-assisted serum metabolite quantification using NMR
    Young-Sang Jung, Jin-Seong Hyeon, Geun-Sook Hwang
    Analytica Chimica Acta vol. 934, 194-202 (2016), doi: 10.1016/j.aca.2016.04.054