Signaling reversing senescence of human diploid fibroblasts by Rg3-20(S)

Study Summary

Ginsenoside Rg3 has 20(S) and 20(R) steteroisomer types. Only Rg3-20(S) restores ATP levels in aged human skin cells and reverses the aging process by increasing NAD+/NADH ratio.

Ginsenoside Rg3-20(S) inhibits mTOR by reducing p53/p21, a cell cycle regulator of aged human skin cells, and inhibits intracellular expression of PI3K.

Molecular and cellular biological research reveals that ginsenoside Rg3-20(S) reverses the aging process through mitochondrial regeneration by regulating PI3K-mTOR-Sirtuin in aged human skin cells for the first time.

[Figure 1] Comparison of SA-β-Gal activity indicating the degree of aging in human skin cells treated with Rg3(S). SA-β-Gal staining in aged human skin cells drops from 80% to 40%.

[Figure 2] In human skin cells treated with Rg3(S), mitochondrial number and NAD+/NADH are restored, and Sirtuin/PGC1α, mitochondrial regeneration signaling are increased.

Scientific evidence for the possible development of red ginseng products enriched with Rg3-20(S) for improving aging is presented by identifying the reverse aging process of Rg3-20(S), one of the main components of red ginseng at the molecular cellular level.