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Development of rapid diagnosis test for COVID-19 2021 > Representative Research Publications > Research Results Home

Development of rapid diagnosis test for COVID-19

  • Biosens. Bioelectron. / March 2021
  • Hye-Yeon Kim(First author), Mi-Jung Kim(First author), Jong-Hwan Lee(First author), Seung-Il Kim(Corresponding author), Chang-Kyun Park(Corresponding author), Hong-Gi Kim(Corresponding author)

Research Summary

In this study, we have developed a SARS-CoV-2 nucleocapsid (NP)-specific rapid diagnosis test(RDT) using the antigen and antibodies we produced.
NP, an important diagnostic antigen produced by replicating virus in respiratory secretions, was cloned and purified for antibody development as soon as the genome sequence of the SARS-CoV-2 was identified.
We produced phage display-derived SARS-CoV-2-specific antibodies and the interaction between antigen and antibodies was examined by ELISA (enzyme-linked immunosorbent assay) and BLI (biolayer interferometry).
As a result, we developed a SARS-CoV-2-specific LFIA (lateral flow immunoassay)-based biosensor for rapid detection of COVID-19 using scFv-Fc antibodies.

Expected Outcomes

We have established the platform of antigen-antibody-based rapid diagnosis for early response in the event of a new emerging infectious disease.

Related Figures

[Figure 1] Scheme showing rapid diagnosis of SARS-CoV-2-specific antigen (nucleocapsid) based on antigen-antibody reaction[Figure 1] Scheme showing rapid diagnosis of SARS-CoV-2-specific antigen (nucleocapsid) based on antigen-antibody reaction

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