Paper-based analytical device for rapid detection of antibiotic-resistant pathogenic bacteria

Research Summary

In this research, we develop a new paper-based multiplex analytical device for simultaneous screening of three analytes (C. difficile toxin A, toxin B, and GDH) for the diagnosis of antibiotic-resistant C. difficile infection (CDI).

The 3-dimensional fluidic path developed in multi-layered papers facilitates the addition and mixing of sample and reagents for signal amplification procedure, resulting in the highly sensitive and accurate detection of CDI with one-step operation (sensitivity of 97%, specificity of 88%, accuracy of 95%).

Based on these analytical performances and versatilities, this device can address the lack of sensitivity, multiplexing, simplicity, and ease-of-use in current EIA and LFA methods, and offers great potential to improve and simplify the current diagnostic workflow for CDI.

Expected Outcomes

This proposed device has potential applications to biomedical, food, water, and environmental testing, as well as other areas requiring rapid and sensitive multiplex detection. It can also be implemented for on-site or point-of-care testing in developing countries and resource-limited settings.

Related Figures

• [Figure 1] Prototype of paper-based multiplex analytical device for CDI diagnosis
• [Figure 2] One-step operating procedure of paper device and CDI testing results