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Representative Research Publications

Development of original technology for the HuNoV binding natural material 2017 > Representative Research Publications > Research Results Home

Exploration of the metal coordination region of concanavalin A for its interaction with human norovirus

  • Journal : Biomaterials (2017. 06.)
  • Equipments : 2D-nano-LC-MS/MS

Authors

Duwoon Kim (1st author, JNU), Hee-Min Lee (1st author, JNU), Kyung-Seo Oh (1st author, JNU), Ah young Ki(CNU, KBSI), Rachael A. Protzman (CNU), Dongkyun Kim (KBSI), Jong-Soon Choi (KBSI), Min Ji Kim (HERI), Sung Hyun Kim (WIKim), Bipin Vaidya (JNU), Seung Jae Lee (JBNU)

Abstract

Rapid methods for the detection and clinical treatment of human norovirus (HuNoV) are needed to control foodborne disease outbreaks. We explore the interactions between HuNoV and concanavalin A (Con A), which could facilitate the development of a sensitive detection tool for HuNoV. Biophysical studies, including hydrogen/deuterium exchange (HDX) mass spectrometry and surface plasmon resonance (SPR), revealed that the metal-coordinated region of Con A is responsible for the observed virus–protein interaction. The mutated carbohydrate binding region of Con A (mCon ACBR) does not affect binding affinity significantly, indicating that MCR of Con A is a major region of interaction with HuNoV (GII.4). The results further contribute to the development of a HuNoV concentration tool, Con A-immobilized polyacrylate beads (Con A-PAB), for rapid detection of genotypes from genogroups I and II (GI and GII). HuNov (GI and GII) can be detected in just 15 min with 90 % recovery through Con A-PAB application. In addition, this method can be used over a wide range of pH values (pH 3.0 - 10.0).

Expected Contribution to Science & Technology

This research has developed a natural material that binds to HuNoV. Based on this materialped a natural material that binds to HuNoVough Con A-PAB application. In addition, thHuNoV.

  • (left) HDX-MS analysis for the interaction regions between Con A and HuNoV (GII.4) HDX-MS analysis for the interaction regions between Con A and HuNoV (GII.4)
  • (center) HDX-MS HDX-MS


  • (right) SPR analysis for interaction between Con A, mutated Con A, and HuNoV (GII.4) SPR analysis for interaction between Con A, mutated Con A, and HuNoV (GII.4)

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