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Roles of NF-KB and ROS in regulation of proinflammatory mediators of inflammation induction in LPS-s 2017 > Representative Research Publications > Research Results Home

The roles of NF-KB and ROS in regulation of proinflammatory mediators of inflammation induction in LPSstimulated zebrafish embryos

  • Fish and Shellfish Immunology (2017.09.)
  • Equipments : Multiphoton Confocal Laser Scanning Microscope (MP-CLSM), Selective Plane Illumination Microscope (SPIM)

Authors

Eun-Yi Ko (first author, KBSI), Su-Hyeon Cho (co-first author, KBSI), Seung-Hae Kwon (KBSI), Chi-Yong Eom (KBSI), Myeong Seon Jeong (KBSI), WonWoo Lee (Jeju National University), Seo-Young Kim (Jeju National University), Soo-Jin Heo (KIOST), Ginnae Ahn (Chonanm National University), Kang Pa Lee (Konkuk University), You-Jin Jeon (co-corresponding author, Jeju National University)

Abstract

To our knowledge, this study is the first to examine this aspect of inflammation; we examined the roles of ROS and NF-KB in zebrafish embryos with inflammation induced by LPS using NAC and PDTC, specific inhibitors of ROS and NF-KB, respectively. Zebrafish embryos treated with LPS had increases in NO, iNOS, COX-2, ROS, and NF-KB compared with the untreated control group. However, NAC and PDTC both inhibited production of NO and expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. In addition, NAC significantly inhibited production of ROS, and PDTC significantly attenuated mRNA expression of NF-KB. Taken together, these results suggest that LPS causes inflammation in zebrafish embryos by increasing inflammatory mediators (NO, iNOS, COX-2) via ROS and NF-KB regulation.

Expected Contribution to Science & Technology

The results of this study provide basic information on the mechanism of action of zebrafish inflammation induction and can be helpful in the screening of candidate functional foods and drugs for the treatment of inflammatory diseases.

  • (left) Effects of NAC on production of ROS and NO and expression of iNOS and COX-2 protein in LPSstimulated zebrafish embryos Effects of NAC on production of ROS and NO and expression of iNOS and COX-2 protein in LPSstimulated zebrafish embryos
  • (right) Effects of PDTC on production of NO and expression of iNOS, COX-2 and NF-KB in LPS-stimulated zebrafish embryos
Effects of PDTC on production of NO and expression of iNOS, COX-2 and NF-KB in LPS-stimulated zebrafish embryos

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